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Pharmed Fish Results from a recent pilot study of the uptake of pharmaceuticals and personal care products (PPCPs) by fish in U.S. rivers have garnered considerable attention since they were pub­ lished online 25 March 2009 ahead of print in Environmental Toxicology and Chemistry. The study, led by Baylor University chemistry pro­ fessor C. Kevin Chambliss and environmental science professor Bryan Brooks, broke new ground by assessing chemical contamination of wild fish at a variety of sampling sites, screening a relatively large and diverse array of PPCPs. The work focused on five rivers in Chicago, Dallas, Orlando, Phoenix, and West Chester (Pennsylvania) that receive waste­ water directly from treatment facilities. All five rivers are considered “effluent­dominated” because the high volume of wastewater they receive continuously exposes their aquatic inhabitants to effluent­derived con­ taminants. “Such [worst­case] expo­ sure scenarios are not represented in standardized toxicity tests used to assess ecological risk of PPCPs to aquatic life,” Chambliss and Brooks point out in their paper. The researchers screened samples of fish fillet (muscle and skin) for 24 pharmaceuticals and 12 personal care products, while samples of fish liver were screened for pharmaceu­ ticals only. The team found levels of 7 pharmaceuticals and 2 personal care products above method detec­ tion limits in the tissues analyzed (see table). This is the first report of the presence of the lipid regulator gem­ fibrozil in U.S. wild fish. The team explained that they chose the study sites because point source discharges from wastewater treatment facilities are “the most significant entry route for human pharmaceuticals into the aquatic envi­ ronment.” However, recent work by Joakim Larsson, an assistant professor at Göteborg University, published in the April 2009 issue of Regulatory Toxicology and Pharmacology suggest pharmaceutical manufacturing plants in the developing world can release much higher quantities into the nearby environment. Larsson says the impact of wastewater from pharmaceutical manufacturers in the developed world is not yet clear, but a 19 April 2009 Associated Press story reports that unpublished data from the U.S. Geological Survey (USGS) and the U.S Environmental Protection Agency (EPA) show U.S. plants also may emit high concen­ trations of pharmaceuticals. In 2005, Chambliss and Brooks were part of the first team to document sub­ stantial uptake of pharmaceuticals in fish, and experts agree their latest findings are consistent with the work conducted to date by a number of scientists. Moreover, the find­ ings have inspired the EPA, which funded the research, to expand its investigation of PPCPs in fish under its National Rivers and Streams Assessment to obtain a more detailed national perspective, says Suzanne Rudzinski, deputy director of the EPA Office of Science and Technology. The agency is investigating whether the concentrations of pharmaceuti­ cals present in fish are of concern for human health or for the fish themselves, she says. The very low concentrations of phar­ maceuticals in the fish flesh may translate into “good news for public health concerns,” says Shane Snyder, a project manager at the Southern Nevada Water Authority’s Applied Research and Development Center. “You’d have to eat tons of fish to come even close to the pharmaceutical levels we predict would be of concern to human health,” he says. He also points out that effluent­dominated waterways constitute an “amazing minority” of U.S. streams. Dana Kolpin, a project chief with the USGS Toxic Substances Hydrology Program, is more cautious, however. “What if these compounds bioaccumulate in tissue or have unintended consequences from the complex mixtures of contaminants we’re exposed to?” he asks. Moreover, he says, “USGS research has shown that PPCPs are commonly present in streams across the United States, not just those that are truly effluent­dominated. While these results in effluent­dominated streams may be the extreme case, this will not be the only setting where such PPCPs will be found in fish tissue.” Last year, Kolpin and colleagues reported in the 15 March 2008 issue of Environmental Science & Technology that earthworms can take up PPCPs from biosolids applied on land. Considered together, the research to date begs for further study into the potential impacts of such chemical mixtures on both aquatic and terrestrial organisms, he says. This is particularly true because researchers’ analytical methods allow them to look for only a few hundred of the thousands of PPCPs that might be in U.S. water bodies, adds Herb Buxton, coordinator for the USGS Toxic Substances Hydrology Program. Comparing personal care prod­ ucts and pharmaceuticals, Snyder says the former may merit greater investigation—some of the concen­ trations documented by Chambliss and Brooks for these compounds are orders of magnitude higher than those seen for pharmaceuticals. For example, the highest uptake reported in the study was of the synthetic fragrance galaxolide, which showed a maximum concentration of 2,100 ng/g in fish fillets (sertraline, Turn on your tap and get hotand cold-running crud.